Glutathione Reconstitution Calculator
A tripeptide antioxidant studied extensively in oxidative-stress and recovery research. This page works out concentration, volume per measured amount, syringe units and preparations per vial for a reconstituted Glutathione preparation, plus the mass-to-molar conversions for its stated molecular weight. Everything runs in your browser; nothing is transmitted or stored.
Also referenced as: GSH
Results
- Concentration
- 2.5 mg/mL (2,500 mcg/mL)
- Per syringe unit
- 25 mcg per unit
- Volume per preparation
- 0.1 mL
- Syringe units to draw
- 10 units
- Preparations per vial
- 20 preparations
Worked example: a 5 mg vial reconstituted with 2 mL of bacteriostatic water. Change any input to recalculate.
A unit-conversion reference for laboratory work. Not a dosing recommendation and not an instruction for human use.
The pre-filled example, step by step
The inputs above are the neutral reference preparation this site uses everywhere the calculator appears: a 5 mg vial, 2 mL of bacteriostatic water, and a 250 mcg target amount. They are not a property of Glutathione and not an amount anyone should draw — they exist so the arithmetic is visible before you change anything.
- Concentration. 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL), which for Glutathione is approximately 8.135 mM at its stated molecular weight.
- Per syringe unit. A U-100 syringe divides 1 mL into 100 units, so one unit holds 2,500 mcg ÷ 100 = 25 mcg.
- Volume for the 250 mcg target. 250 mcg ÷ 2,500 mcg/mL = 0.1 mL, or 10 units on that syringe.
- Preparations per vial. 5,000 mcg ÷ 250 mcg = 20 preparations.
Arithmetic on a laboratory preparation, shown as a unit-conversion reference. Not medical advice, not a dosing recommendation, not an instruction for human use. The full derivation, and why the diluent volume is a choice rather than a constant, is on the main reconstitution calculator.
Mass and molar equivalents for Glutathione
Derived from the molecular weight recorded on the Glutathione entry: 307.32 g/mol. Concentration in molar units is what an in-vitro protocol is usually specified in.
| Quantity | Molar equivalent |
|---|---|
| 1 mg of Glutathione | 3,254 nmol (3.254 µmol) |
| 1 mcg of Glutathione | 3.254 nmol |
| A 1 mg/mL solution | 3.254 mM (3,254 µM) |
Molar amount = mass ÷ molecular weight. Figures are shown to four significant digits and are a unit conversion, not a measurement.
Reconstitution recorded for Glutathione
Supplied as reduced-glutathione powder or solution; injectable forms are dissolved in sterile diluent per protocol rather than bacteriostatic water for a lyophilized research peptide
Quoted from the Glutathione entry. Amounts reported in the research literature for this compound are documented on that entry, under the disclaimer they belong with — they are not reproduced next to a calculator. Read the full Glutathione entry.
Handling questions for Glutathione
Why does the gamma peptide bond in glutathione matter?
The gamma linkage between glutamate and cysteine is not the standard alpha peptide bond, which makes glutathione resistant to cleavage by ordinary peptidases and contributes to its intracellular stability. Only the enzyme gamma-glutamyl transpeptidase can break this bond, which is central to how glutathione is recycled and transported.
Answers are published on the Glutathione entry. Educational summaries of research-literature context, not medical advice.
Continue exploring
- Full Glutathione entry — Endogenous antioxidant tripeptide
- Reconstitution calculator — the maths in full, with a second worked example
- Storage & Handling guide
- Bacteriostatic water — what it is and how it differs from sterile water
- Compare Glutathione with another library entry