PE-22-28 Reconstitution Calculator
A synthetic peptide analog of spadin referenced in mood and neuroplasticity research. This page works out concentration, volume per measured amount, syringe units and preparations per vial for a reconstituted PE-22-28 preparation, plus the mass-to-molar conversions for its stated molecular weight. Everything runs in your browser; nothing is transmitted or stored.
Also referenced as: PE22-28, Spadin analogue
Results
- Concentration
- 2.5 mg/mL (2,500 mcg/mL)
- Per syringe unit
- 25 mcg per unit
- Volume per preparation
- 0.1 mL
- Syringe units to draw
- 10 units
- Preparations per vial
- 20 preparations
Worked example: a 5 mg vial reconstituted with 2 mL of bacteriostatic water. Change any input to recalculate.
A unit-conversion reference for laboratory work. Not a dosing recommendation and not an instruction for human use.
The pre-filled example, step by step
The inputs above are the neutral reference preparation this site uses everywhere the calculator appears: a 5 mg vial, 2 mL of bacteriostatic water, and a 250 mcg target amount. They are not a property of PE-22-28 and not an amount anyone should draw — they exist so the arithmetic is visible before you change anything.
- Concentration. 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL), which for PE-22-28 is approximately 3.23 mM at its stated molecular weight.
- Per syringe unit. A U-100 syringe divides 1 mL into 100 units, so one unit holds 2,500 mcg ÷ 100 = 25 mcg.
- Volume for the 250 mcg target. 250 mcg ÷ 2,500 mcg/mL = 0.1 mL, or 10 units on that syringe.
- Preparations per vial. 5,000 mcg ÷ 250 mcg = 20 preparations.
Arithmetic on a laboratory preparation, shown as a unit-conversion reference. Not medical advice, not a dosing recommendation, not an instruction for human use. The full derivation, and why the diluent volume is a choice rather than a constant, is on the main reconstitution calculator.
Mass and molar equivalents for PE-22-28
Derived from the molecular weight recorded on the PE-22-28 entry: 773.9 g/mol. Concentration in molar units is what an in-vitro protocol is usually specified in.
| Quantity | Molar equivalent |
|---|---|
| 1 mg of PE-22-28 | 1,292 nmol (1.292 µmol) |
| 1 mcg of PE-22-28 | 1.292 nmol |
| A 1 mg/mL solution | 1.292 mM (1,292 µM) |
Molar amount = mass ÷ molecular weight. Figures are shown to four significant digits and are a unit conversion, not a measurement.
Reconstitution recorded for PE-22-28
Lyophilized peptide typically reconstituted with bacteriostatic water for research handling
Quoted from the PE-22-28 entry. Amounts reported in the research literature for this compound are documented on that entry, under the disclaimer they belong with — they are not reproduced next to a calculator. Read the full PE-22-28 entry.
Handling questions for PE-22-28
How does PE-22-28 relate to spadin?
Spadin is a fragment derived from the propeptide of the sorting receptor sortilin, and PE-22-28 is a shortened, optimized fragment of it. Research describes PE-22-28 as among the smallest spadin fragments that retains TREK-1 inhibitory activity, with improved stability over the parent peptide.
Answers are published on the PE-22-28 entry. Educational summaries of research-literature context, not medical advice.
Continue exploring
- Full PE-22-28 entry — Spadin-derived synthetic peptide (TREK-1 channel blocker)
- Reconstitution calculator — the maths in full, with a second worked example
- Storage & Handling guide
- Bacteriostatic water — what it is and how it differs from sterile water
- Compare PE-22-28 with another library entry