PeptiDex
Research Tool

TB-500 Reconstitution Calculator

A synthetic fragment of Thymosin Beta-4 frequently studied alongside BPC-157 in repair-related research. This page works out concentration, volume per measured amount, syringe units and preparations per vial for a reconstituted TB-500 preparation. Everything runs in your browser; nothing is transmitted or stored.

Also referenced as: Thymosin Beta-4

Actin-binding peptide fragment
For research and educational purposes only. This is a unit-conversion tool for laboratory reference. It is not medical advice, not a dosing recommendation, and not an instruction for human use.

Inputs

Results

Concentration
2.5 mg/mL (2,500 mcg/mL)
Per syringe unit
25 mcg per unit
Volume per preparation
0.1 mL
Syringe units to draw
10 units
Preparations per vial
20 preparations

Worked example: a 5 mg vial reconstituted with 2 mL of bacteriostatic water. Change any input to recalculate.

A unit-conversion reference for laboratory work. Not a dosing recommendation and not an instruction for human use.

The pre-filled example, step by step

The inputs above are the neutral reference preparation this site uses everywhere the calculator appears: a 5 mg vial, 2 mL of bacteriostatic water, and a 250 mcg target amount. They are not a property of TB-500 and not an amount anyone should draw — they exist so the arithmetic is visible before you change anything.

  1. Concentration. 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL).
  2. Per syringe unit. A U-100 syringe divides 1 mL into 100 units, so one unit holds 2,500 mcg ÷ 100 = 25 mcg.
  3. Volume for the 250 mcg target. 250 mcg ÷ 2,500 mcg/mL = 0.1 mL, or 10 units on that syringe.
  4. Preparations per vial. 5,000 mcg ÷ 250 mcg = 20 preparations.

Arithmetic on a laboratory preparation, shown as a unit-conversion reference. Not medical advice, not a dosing recommendation, not an instruction for human use. The full derivation, and why the diluent volume is a choice rather than a constant, is on the main reconstitution calculator.

Molecular weight recorded for TB-500

~4921 Da (full-length thymosin beta-4); ~889 Da for the Ac-LKKTETQ fragment form

No single molar equivalent is derived here: the TB-500 entry does not record one unambiguous molecular weight, and choosing between the figures it does record would be a guess. Convert against whichever mass matches the material on your Certificate of Analysis.

Reconstitution recorded for TB-500

Lyophilized; reconstituted with bacteriostatic water, swirled not shaken; store reconstituted at 2-8 C protected from light

Quoted from the TB-500 entry. Amounts reported in the research literature for this compound are documented on that entry, under the disclaimer they belong with — they are not reproduced next to a calculator. Read the full TB-500 entry.

Handling questions for TB-500

How is TB-500 reconstituted and stored?

It is supplied lyophilized and reconstituted with bacteriostatic water, added gently and swirled rather than shaken. Reconstituted solution is stored refrigerated at 2-8 C and protected from light, while lyophilized powder is more stable and can be frozen for longer-term storage.

Answers are published on the TB-500 entry. Educational summaries of research-literature context, not medical advice.

Continue exploring