Research Tool

VIP Reconstitution Calculator

Vasoactive intestinal peptide, a 28-amino-acid neuropeptide studied across vascular and neurological research. This page works out concentration, volume per measured amount, syringe units and preparations per vial for a reconstituted VIP preparation, plus the mass-to-molar conversions for its stated molecular weight. Everything runs in your browser; nothing is transmitted or stored.

Also referenced as: Vasoactive Intestinal Peptide

Neuropeptide hormoneMW ~3326 g/molt½ ~1-2 minutes (native VIP, plasma)
For research and educational purposes only. This is a unit-conversion tool for laboratory reference. It is not medical advice, not a dosing recommendation, and not an instruction for human use.

Inputs

Results

Concentration
2.5 mg/mL (2,500 mcg/mL)
Per syringe unit
25 mcg per unit
Volume per preparation
0.1 mL
Syringe units to draw
10 units
Preparations per vial
20 preparations

Worked example: a 5 mg vial reconstituted with 2 mL of bacteriostatic water. Change any input to recalculate.

A unit-conversion reference for laboratory work. Not a dosing recommendation and not an instruction for human use.

The pre-filled example, step by step

The inputs above are the neutral reference preparation this site uses everywhere the calculator appears: a 5 mg vial, 2 mL of bacteriostatic water, and a 250 mcg target amount. They are not a property of VIP and not an amount anyone should draw — they exist so the arithmetic is visible before you change anything.

  1. Concentration. 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL), which for VIP is approximately 0.7517 mM at its stated molecular weight.
  2. Per syringe unit. A U-100 syringe divides 1 mL into 100 units, so one unit holds 2,500 mcg ÷ 100 = 25 mcg.
  3. Volume for the 250 mcg target. 250 mcg ÷ 2,500 mcg/mL = 0.1 mL, or 10 units on that syringe.
  4. Preparations per vial. 5,000 mcg ÷ 250 mcg = 20 preparations.

Arithmetic on a laboratory preparation, shown as a unit-conversion reference. Not medical advice, not a dosing recommendation, not an instruction for human use. The full derivation, and why the diluent volume is a choice rather than a constant, is on the main reconstitution calculator.

Mass and molar equivalents for VIP

Derived from the molecular weight recorded on the VIP entry: ~3326 g/mol — the entry states this as an approximate figure, so the equivalents below are approximate too. Concentration in molar units is what an in-vitro protocol is usually specified in.

Mass to molar conversions for VIP at its stated molecular weight.
QuantityMolar equivalent
1 mg of VIP300.7 nmol (0.3007 µmol)
1 mcg of VIP0.3007 nmol
A 1 mg/mL solution0.3007 mM (300.7 µM)

Molar amount = mass ÷ molecular weight. Figures are shown to four significant digits and are a unit conversion, not a measurement.

Reconstitution recorded for VIP

Supplied lyophilized; reconstituted in sterile water or buffer for research use and kept cold due to rapid degradation.

Quoted from the VIP entry. Amounts reported in the research literature for this compound are documented on that entry, under the disclaimer they belong with — they are not reproduced next to a calculator. Read the full VIP entry.

Handling questions for VIP

How is VIP handled and stored?

VIP is typically supplied as a lyophilized powder, reconstituted before use, and is sensitive to degradation. Lyophilized peptide is kept frozen and protected from light; reconstituted solution is refrigerated and used quickly given the peptide's instability. A COA with HPLC purity and mass-spec identity is standard to expect.

Answers are published on the VIP entry. Educational summaries of research-literature context, not medical advice.

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